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Journal: Current biology : CB

Article Title: Gap junction coupling shapes the encoding of light in the developing retina

doi: 10.1016/j.cub.2019.10.025

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti GFP Thermo Fisher Scientific Cat# A-11122; RRID: AB_221569 Mouse monoclonal anti SMI32 Millipore Cat# NE1023-100UL; RRID: AB_10682557 Donkey anti rabbit Alexa Fluor 488 Molecular Probes Cat# A-21206, RRID:AB_141708 Goat anti mouse Alexa Fluor 647 Molecular Probes Cat# A-21235, RRID:AB_141693 Bacterial and Virus Strains Biological Samples Chemicals, Peptides, and Recombinant Proteins Vectashield Vector Laboratories Cat# H-1400, RRID:AB_2336787 Cal-590 AM AAT Bioquest Cat# 20510 Cal-590 potassium salt AAT Bioquest Cat# 20518 Neurobiotin Vector Laboratories Cat# SP-1120, RRID:AB_2313575 Streptavidin Alexa Fluor 594 conjugate Thermo Fisher Scientific Cat# {"type":"entrez-protein","attrs":{"text":"S11227","term_id":"94022","term_text":"pir||S11227"}} S11227 QX 314 bromide Tocris Cat# 1014 Di-hydro-β-erythroidine (DHβE) Tocris Cat# 2349 Meclofenamic acid (MFA) Sigma Aldrich Cat# M4531-1G SCH23390 Hydrochloride (SCH23390) Tocris Cat# 0925 D-2-amino-5-phosphonovalerate (D-AP5) Tocris Cat# 0106 6,7-Dinitroquinoxaline-2,3-dione (DNQX) Tocris Cat# 0189/10 Critical Commercial Assays Deposited Data Calcium imaging of light responses in the neonatal retina This paper https://data.mendeley.com/datasets/ddsj9pjhf6 Experimental Models: Cell Lines Experimental Models: Organisms/Strains Opn4 eGFP mice [ 7 ] Paulo Kofuji Opn4 Cre mice [ 53 ] Tiffany Schmidt Drd1 tm2.1Stl mice Jax Cat# JAX:025700, RRID:IMSR_JAX:025700 C57Bl/6J Jax Cat# JAX:000664, RRID:IMSR_JAX:000664 Oligonucleotides Recombinant DNA Software and Algorithms ScanImage 4 [ 56 ] http://scanimage.vidriotechnologies.com/display/SIH/ScanImage+Home RRID:SCR_014307 Clampex 10.3 Molecular Devices RRID:SCR_011323 MATLAB 2019a MathWorks https://www.mathworks.com/products/matlab.html RRID:SCR_001622 FIJI [ 57 ] https://imagej.net/ RRID:SCR_003070 Calcium imaging analysis and functional clustering code This paper https://github.com/FellerLabCodeShare/ipRGC-development-project SpaSM [ 59 ] http://www2.imm.dtu.dk/projects/spasm/ Other Open in a separate window KEY RESOURCES TABLE Six functional groups of light-responsive cells correspond to mixed ipRGC subtypes Gap junctions transmit slow photocurrents and spikelets Blocking gap junctions reduces light sensitivity in most functional groups Increased gap junction coupling enhances light sensitivity of all functional groups

Techniques: Virus, Recombinant, Plasmid Preparation, Imaging, Software, Functional Assay